Assays developed by our laboratory
- D-loop capture (DLC) and extension (DLE) assays:
- Detection of multi-invasion-induced rearrangements (MIR):
- D-loop mapping assay (DMA):
Yeast protocols
- Basics on Saccharomyces cerevisiae
- General tips
- DNA extraction using CTAB
- DNA extraction using PCIA
- DNA extraction in agarose plugs (for Pulse-Field gel)
- Gene expression induction with Galactose (like the HO endonuclease with the GAL-HO construct)
- Immunoprecipitation
- Irradiation (gamma-ray)
- Media recipes
- MMS handling
- PCR on colony (fast transformant screening)
- Plasmid transfer from E. coli to S. cerevisiae
- Proteins extraction using TCA
- Psoralen crosslinking and DNA extraction
- Psoralen crosslinking (variation very severe crosslinking density)
- Psoralen de-crosslinking
- Restriction enzyme analysis of plasmids
- Yeast sporulation
- Yeast spotting assay
- Yeast strains archiving
- Yeast transformation / Fast yeast transformation
Biochemistry protocols
- DNA strand exchange reaction (D-loop assay)
- ATPase assay
- Nucleoprotein gel assay for Rad51-DNA complexes
- Fermentor protocol
Molecular biology protocols
- Ligation (sticky ends)
- Ligation (blunt ends)
- DNA precipitation with Ethanol and salts
- Dialysis tube preparation
- Dephosphorylation of plasmid
- Denaturing purification of insoluble proteins
- Lab Solutions
Gel electrophoresis
- Antibody production
- Western blot
- Southern blot
- 2D-gel
- Protein polyacrylamide gel
- DNA polyacrylamide gel
- Pulse-Field gel
E. coli protocols